Chinese Journal of Computers   Full Text
Title	Development and Application of Fluorescence Technology in DNA Computing
Authors	ZHANG Cheng YANG Jing WANG Shu-Dong
Address	(Key Laboratory of High Confidence Software Technologies of Ministry of Education, School of Electronic Engineering and Computer Science, Peking University, Beijing 100871)
Year	2009
Issue	No.12(2300—2310)
Abstract &   Background	Abstract DNA computing, as a focus of scientific fields, has developed from simple to complex, from theory to application. In this course, the fluorescence, which can be handled easily and has a sensitive respond, plays an important role. This paper pays more attentions to DNA computing and fluorescence. On one hand, major applications of fluorescence in DNA computing are summed up. There are several aspects: (1)surface computing labeled by fluorescence. (2)The fluorescence detecting combined with enzyme cutting. (3)The fluorescence technology used in DNA hybridization and releasing. (4)The fluorescence logical gate based on RNAi. At first, the expression of fluorescence proteins is controlled by RNAi. (5)The fluorescence technology used in DNA self-assembly. (6)The fluorescence technology based on DNA transforming. On the other hand, several novel methods of fluorescence are introduced as following: (1)Signal amplification of fluorescence molecular beacons. (2)The fluorescence technology associated with magnetic beads. (3)The fluorescence technology changing with PH. (4)Detecting miRNAs with fluorescence technology. Only combine these two aspects into whole, most of the advantages of DNA computing can be taken used entirely. Keywords DNA computing; fluorescence labeling; nanotechnology; DNA molecule; hybridization Background This research is supported by the National Natural Science Foundation of China (Grant Nos.60533010，30670540，60874036, and 60503002), the National High Technology Research and Development Program (863 Program) (Grant No.2006AA01Z104), the Ph.D. Programs Foundation of the Ministry of Education of China (Grant No.20070001020), and the Postdoctoral Science Foundation of China (Grant No.20060400344). DNA computing is a new research focus relating to computer science, engineering, biology, chemistry and mathematics. This paper introduces the applications of fluorescence in DNA computing. DNA computing asks more accurate quality, rational steps and complicated component. Fluorescence technology can meet these requirements. As a new research focus, recently more papers about DNA computing are published in high level research journals, like science and nature. Using fluorescence, it is easier to label single molecule and detect signals. Therefore, fluorescence technology will promote the development of DNA computing. Meanwhile, fluorescence technology also enlarges its applications. Such knowledge fusion is the elementary promote in science.